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pbluescript ii ks vector  (New England Biolabs)


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    Structured Review

    New England Biolabs pbluescript ii ks vector
    Pbluescript Ii Ks Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 332 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript+ks++vector/NcoI-HF/pmc10944469-177-13-22
    Average 97 stars, based on 332 article reviews
    pbluescript ii ks vector - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: High throughput assay for compounds that boost BDNF expression in neurons
    Article Snippet: .. The DNA donor plasmid contains two homology arms flanking the NLuc sequence, which were cloned into BamHI digested pBluescript II KS (−) vector using the Gibson assembly method (NEB, E5510). ..

    Article Title: Genetic Dissection of BDNF and TrkB Expression in Glial Cells
    Article Snippet: .. The DNA donor plasmid contains two homology arms with 840 bp flanking the P2A-Cre sequence, which were cloned into BamHI-digested pBluescript II KS (-) vector using the Gibson assembly method (NEB, Ipswich, MA, USA, E5510). .. To block further Cas9 targeting and recutting after undergoing homology-direct repair, we introduced a silent mutation into the PAM motif of the sgRNA located within the 3′ homology arm in the donor plasmid by using Q5 Site-Directed Mutagenesis kit (NEB, E0054).

    Article Title: Genetic Dissection of BDNF and TrkB Expression in Glial Cells.
    Article Snippet: .. The DNA donor plasmid contains two homology arms with 840 bp flanking the Biomolecules 2024, 14, 91 3 of 18 P2A-Cre sequence, which were cloned into BamHI-digested pBluescript II KS (-) vector using the Gibson assembly method (NEB, Ipswich, MA, USA, E5510). .. To block further Cas9 targeting and recutting after undergoing homology-direct repair, we introduced a silent mutation into the PAM motif of the sgRNA located within the 3′ homology arm in the donor plasmid by using Q5 Site-Directed Mutagenesis kit (NEB, E0054).

    Sequencing:

    Article Title: High throughput assay for compounds that boost BDNF expression in neurons
    Article Snippet: .. The DNA donor plasmid contains two homology arms flanking the NLuc sequence, which were cloned into BamHI digested pBluescript II KS (−) vector using the Gibson assembly method (NEB, E5510). ..

    Article Title: Genetic Dissection of BDNF and TrkB Expression in Glial Cells
    Article Snippet: .. The DNA donor plasmid contains two homology arms with 840 bp flanking the P2A-Cre sequence, which were cloned into BamHI-digested pBluescript II KS (-) vector using the Gibson assembly method (NEB, Ipswich, MA, USA, E5510). .. To block further Cas9 targeting and recutting after undergoing homology-direct repair, we introduced a silent mutation into the PAM motif of the sgRNA located within the 3′ homology arm in the donor plasmid by using Q5 Site-Directed Mutagenesis kit (NEB, E0054).

    Article Title: Genetic Dissection of BDNF and TrkB Expression in Glial Cells.
    Article Snippet: .. The DNA donor plasmid contains two homology arms with 840 bp flanking the Biomolecules 2024, 14, 91 3 of 18 P2A-Cre sequence, which were cloned into BamHI-digested pBluescript II KS (-) vector using the Gibson assembly method (NEB, Ipswich, MA, USA, E5510). .. To block further Cas9 targeting and recutting after undergoing homology-direct repair, we introduced a silent mutation into the PAM motif of the sgRNA located within the 3′ homology arm in the donor plasmid by using Q5 Site-Directed Mutagenesis kit (NEB, E0054).

    Clone Assay:

    Article Title: High throughput assay for compounds that boost BDNF expression in neurons
    Article Snippet: .. The DNA donor plasmid contains two homology arms flanking the NLuc sequence, which were cloned into BamHI digested pBluescript II KS (−) vector using the Gibson assembly method (NEB, E5510). ..

    Article Title: Genetic Dissection of BDNF and TrkB Expression in Glial Cells
    Article Snippet: .. The DNA donor plasmid contains two homology arms with 840 bp flanking the P2A-Cre sequence, which were cloned into BamHI-digested pBluescript II KS (-) vector using the Gibson assembly method (NEB, Ipswich, MA, USA, E5510). .. To block further Cas9 targeting and recutting after undergoing homology-direct repair, we introduced a silent mutation into the PAM motif of the sgRNA located within the 3′ homology arm in the donor plasmid by using Q5 Site-Directed Mutagenesis kit (NEB, E0054).

    Article Title: Protein kinase C activity modulates nuclear Lamin A/C dynamics in HeLa cells
    Article Snippet: .. This region was then cloned into the LMNA translation start site in the pBlueScript II KS (−) vector after digestion with NcoI-HF (New England BioLabs, R3193S). ..

    Article Title: Genetic Dissection of BDNF and TrkB Expression in Glial Cells.
    Article Snippet: .. The DNA donor plasmid contains two homology arms with 840 bp flanking the Biomolecules 2024, 14, 91 3 of 18 P2A-Cre sequence, which were cloned into BamHI-digested pBluescript II KS (-) vector using the Gibson assembly method (NEB, Ipswich, MA, USA, E5510). .. To block further Cas9 targeting and recutting after undergoing homology-direct repair, we introduced a silent mutation into the PAM motif of the sgRNA located within the 3′ homology arm in the donor plasmid by using Q5 Site-Directed Mutagenesis kit (NEB, E0054).

    CRISPR:

    Article Title: Methods, cells and organisms
    Article Snippet: .. To reconstruct the CRISPR/Cas system described in Cong et al the above DNA fragments in addition to EcoRV linearised pBlueScript KS+ vector will be assembled using Gibson Assembly kit (NEB Cat No. E5510 S). ..



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